Hatay İli Biber (Capsicum Annuum L.) Üretim Alanlarında Fitoplazma Hastalıklarının, Potansiyel Vektörlerinin ve Diğer Konukçularının Araştırılması
نویسندگان
چکیده
Aims: This study was conducted to determine the phytoplasma diseases of pepper plants in Hatay province between 2016 and 2019.Methods Results: Samples (Capsicum annuum), sesame (Sesamum indicum), basil (Ocimum basilicum), tomato (Solanum lycopersicum) bindweed (Convolvulus arvensis) which had typical symptoms insect (Cicadellidae spp.) samples were collected from fields. Empoasca sp., Cicadulina bipunctata, Psammotettix Balchutha hebe, Euscelidius Anaceratagallia laevis Exitianus capicola found as potential vectors phytoplasmas descending order population densities. T-budding grafting technique successful for transmission infected healthy periwinkle (Catharanthus roseus) controlled conditions. Phytoplasmas detected pepper, tomato, basil, some samples. Total nucleic acid isolation accomplished by CTAB method. Direct Nested PCR used employing R16F1/R16R0 R16F2n/R16R2 primer pairs respectively.Conclusions: DNA positive sequenced, uploaded Genbank, identified Ca. Phytoplasma trifolii on (MT993358), (MT994434), (MT992754), (MT994432), sp. (MT994430), (MT994433), (MT994431); solani binweed (MT993422) (MT992796).Significance Impact Study: According our knowledge, this is first identify upload Genbank Turkey.
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ژورنال
عنوان ژورنال: Mustafa Kemal U?niversitesi tar?m bilimleri dergisi
سال: 2022
ISSN: ['2667-7733']
DOI: https://doi.org/10.37908/mkutbd.1060097